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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has actually been noticed in several tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was uncovered to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential purpose in advertising mobile-cycle development in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been described. Two added splice variants were described but detailed nucleotide sequence information and facts is just not yet readily available.
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Blocking peptides are peptides that bind specially to the goal antibody and block antibody binding. These peptide ordinarily consists of the epitope recognized with the antibody.
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This manual outlines even further doable optimization of cellular and immunoassay parameters to make sure the very best effects are...
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This guideline outlines additional probable optimization of cellular and immunoassay parameters to make certain the absolute best final results are...
Antibodies sure to the blocking peptide now not bind into the epitope on the concentrate on protein. By evaluating the staining with the blocked antibody vs . the antibody alone, you can see which staining is distinct.
Goal Details Cyclin E belongs for the extremely conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance with the cell cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins show unique expression and degradation patterns which lead into the temporal coordination of every mitotic function. Cyclin E forms a posh with and features like a regulatory subunit of CDK2, whose action is needed for mobile cycle G1/S transition. This protein accumulates within the G1-S phase boundary and is degraded as cells development via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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